Promoter dna replication

Gourse RL, Ross W, Gaal T. UPs and downs in bacterial transcription initiation: the role of the α subunit of RNA polymerase in promoter recognition. J Biol Chem. 2000;5:613–626. [PMC free article: PMC24504] [PubMed: 9843944]

Gartenberg MR, Crothers DM.

Synthetic DNA bending sequences increase the rate of in vitro transcription initiation at the Escherichia coli lac promoter. A comparison between the lac wild-type and the UV5 sites of Escherichia coli. Interaction site of Escherichia coli cyclic AMP receptor protein on DNA of galactose operon promoters. [PMC free article: PMC282158] [PubMed: 3174629]

Lozinski T, Adrych-Rozek K, Markiewicz WT.

et al. EMBO J. 1990;9:3733–3742. However, for these experiments, one must have a protein candidate to test and is not able to identify unknown proteins bound to a particular promoter. Analysis of sequence elements important for the synthesis and control of ribosomal RNA in E. coli. 2003;326:1361–1372. This unwinding creates a “transcription bubble,” exposing the gene’s template strand so RNA polymerase can begin synthesizing an RNA molecule.

In prokaryotes, the initiation is more direct.

Binding of the arginine repressor of Escherichia coli K-12 to its operator sites.

1995;253:32–38. 1993;232:530–554. Mol Gen Genet. If a mutation causes a gene to be overexpressed, it might contribute to conditions like cancer, which involves uncontrolled cell growth. An upstream activating sequence containing curved DNA involved in activation of the Clostridium perfringens plc promoter. J Mol Biol. J Mol Biol.